Fibroblast growth factor binding to perlecan isolated  from the growth plate

Abstract 2005 MHE Conference

Leigh Westa, Prasanthi Govindraja, Xiuqin Zhangb, David M. Ornitz(b), John R. Hassell, (a)Center for Research in
Skeletal Development and  Pediatric Orthopaedics,
Shriners Hospitals for Children, Tampa, FL 33612   
(b)Department of Molecular Biology and Pharmacology, Washington  University Medical School,660 S. Euclid
Avenue, St. Louis, MO 63310,
(c)Department of Biochemistry and Molecular Biology,College of  Medicine, University of  South Florida, Tampa, FL

Fibroblast growth factor (FGF)-2, FGF-9 and FGF-18 are three FGF's that have been shown to regulate chondrocyte proliferation
in the growth plate.  

Heparin and heparan sulfate proteoglycans from endothelial cells have been shown to bind FGF-2. Perlecan is present in the
growth plate as a proteoglycan containing both heparan and chondroitin sulfate chains and the phenotype of the perlecan
knockout mouse shows it is necessary for the proliferation of chondrocytes in the growth plate.

We evaluated the binding of FGF's to perlecan from the growth plate using a cationic filtration assay.  We found FGF-2 bound
primary to the heparan sulfate chains on perlecan but that the core protein was also involved in FGF-2 binding when the
heparan sulfate chains were present.  Removal of Chondroitin sulfate chains on perlecan enhanced FGF-2 binding slightly.  
FGF-1, FGF-9 and FGF-18 showed no binding to perlecan but FGF-7 showed a low level of binding.  

We also found, using recombinant FGF receptors in a separate capture assay that while the receptors would bind to a
heparin-FGF-2 complex, the receptors would not bind to the perlecan-FGF-2 complex.  Similarly, perlecan did not augment
FGF-2 stimulation of [3H]-thymine incorporation in BaF3 cells.   

These data show perlecan can bind to FGF-2 by its heparan sulfate chains but that chondroitin sulfate chains on the perlecan
acts to block transfer of the bound FGF-2 to the receptor.
Research authored by Dr. Hassell
Click the tab and a window will appear.
List of Publications via PubMed
(NIH National Library of Medicine)
Dr. Hassell's research
Home page
Be notified of page updates
it's private

Site Map
Wings of HOPE as we REACH
for the CURE to
Multiple Hereditary Exostoses / Multiple

powered by FreeFind
Website Search
You need Java to see this applet.
The MHE Research Foundation, we comply with the HONcode standard for health trust worthy information: By the Health On the Net Foundation.      
here to verify.# HON Conduct 282463  and is the patient support link on the US Government Genetics Home Reference (
website, also linked for Patient Information on
The Diseases Database a cross-referenced index of human disease, as well as the
Intute: health & life sciences  a free online service  providing access to the very best Web resources for education and research located in the  UK
The MHE Research Foundation is proud to be working with the EuroBoNeT consortium, a European Commission granted Network of Excellence for
studying the pathology and genetics of bone tumors.
This website is regularly reviewed by members of the Scientific and Medical Advisory Board of the MHE Research Foundation.

All online submission forms use (SSL AES 256 bit encryption (High); RSA 1024 bit exchange) Protocol with
Privacy protection.
Our goal is to make this website as safe and user friendly as possible.
The MHE Research Foundation is a participating member organization of the
United States Bone and Joint Decade,  (USBJD) & the  USBJD Rare Bone Disease Patient Network
number of users on this website page now :

Written consent must be obtained to attach web pages or the files attached to this website, please email the webmaster.

Email the webmaster:
Materials on this website are protected by copyright
Copyright © 2009 The MHE Research Foundation

Disclaimer:   While many find the information useful, it is in no way a substitute for professional medical care.
The information provided here is for educational and informational purposes only. This website does not engage in the practice of medicine.
In all cases we recommend that you consult your own physician regarding any course of treatment or medicine.

This web page was updated last on 12/16/09, 4:0O pm Eastern time
The MHE Research Foundation is proud to be an affiliate of the Society For Glycobiology
The MHE Research Foundationis proud to be a partnering society with ASMB & CTOS